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Immunoassay for Transthyretin Variants associated with Amyloid Neuropathy
Author(s) -
COSTA P. M. P.,
TEIXEIRA A.,
SARAIVA M. J. M.,
COSTA P. P.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01710.x
Subject(s) - transthyretin , polyneuropathy , epitope , immunogen , amyloid polyneuropathy , monoclonal antibody , peripheral neuropathy , amyloidosis , immunoassay , amyloid (mycology) , recombinant dna , antibody , tetramer , monoclonal , medicine , microbiology and biotechnology , chemistry , immunology , biology , pathology , biochemistry , disease , endocrinology , gene , enzyme , diabetes mellitus , age of onset
An anti‐transthyretin (TTR) mouse monoclonal antibody (88.6.FD6)of IgG1 subclass, obtained using as immunogen TTR from the serum of a patient with familial amyloidotic polyneuropathy, was found to bind to sera from carriers of several amyloidogenic TTR variants associated with peripheral neuropathy, but not to normal sera or sera from carriers of non‐pathogenic or cardiomyopathic variants, in an ELISA performed under special conditions. Further characterization suggests that it recognizes an epitope near the N‐terminal side of the TTR monomer. We propose that this epitope is exposed in amyloid and could be implicated in fibril deposition in the peripheral nervous system.