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Lymphocyte‐Endothelial Interactions in Inflamed Synovia:Involvement of Several Adhesion Molecules and Integrin Epitopes
Author(s) -
FISCHER C.,
THIELE H.G.,
HAMANN A.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01708.x
Subject(s) - lymphocyte homing receptor , integrin , cell adhesion molecule , chemistry , rheumatoid arthritis , lymphocyte , adhesion , immunology , cell adhesion , endothelium , selectin , microbiology and biotechnology , soluble cell adhesion molecules , homing (biology) , receptor , medicine , biology , biochemistry , ecology , organic chemistry
The role of several adhesion molecules for lymphocyte endothelial interactions in the synovia of rheumatoid arthritis patients was studied using the frozen section assay. Partial inhibition of lymphocyte binding to endothelium of synovial sections could be observed with antibodies against CD44, L‐selectin, and β 1 ‐ and β 2 ‐integrins, pointing to the participation of several adhesion molecules in the regulation of lymphocyte immigration into inflamed synovia rather than the presence of a unique homing receptor. Different degrees of inhibition were found within a series of antibodies against α 4 and β 1 ‐integrins known to have functional effects in other interaction systems. In addition, increased binding to endothelial cells was induced when lymphocytes were pretreated with TS2/16 anti‐β 1 IgG, whereas binding to non‐endothelial components of synovia was increased after treatment with HP 2/4 (anti‐α 4 ) Fab. The data suggest a multifunctional role of α/β 1 ‐integrins in directly mediating adhesion as well as regulating adhesive interactions in the rheumatoid synovia.