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Secretion of IL‐2, IL‐3, IL‐4, IL‐6 and GM‐CSF by CD4 + and CD8 + TCRαβ + T‐Cell Clones derived early after Allogeneic Bone Marrow Transplantation
Author(s) -
BRUSERUD Ø.,
EHNINGER G.,
HAMANN W.,
PAWELEC G.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01695.x
Subject(s) - autocrine signalling , cd8 , cytokine , biology , bone marrow , t cell , secretion , granulocyte macrophage colony stimulating factor , immunology , transplantation , interleukin , interleukin 4 , interleukin 2 , medicine , endocrinology , immune system , cell culture , genetics
Secretion of different cytokines may be an important T‐cell effector mechanism for bone marrow engraftment, graft versus host disease and graft versus leukaemia effects after allogeneic bone marrow transplantation (BMT). Cytokine secretion and autocrine proliferative capacity of T‐cell clones derived from leukaemia patients 3–6 weeks after allogeneic bone marrow transplantation were investigated. Only a minority of post‐transplant T‐cell clones (23/120; 19%) was capable of undergoing autocrine proliferation. By contrast, 21/65 (32%) normal control clones from the marrow donors derived under the same conditions were autocrine proliferative. All clones were interleukin‐2 (IL‐2) responsive. A majority (12/17; 71%) of autocrine proliferating post‐transplant clones secreted detectable IL‐2. Compared with control clones, CD4 + T‐cell clones derived early after BMT produced decreased levels of interleukin‐4 (IL‐4) and interleukin‐6 (IL‐6), whereas secretion of interleukin‐3 (IL‐3) and granulocyte/macrophage colony‐stimulating factor (GM‐CSF) showed no significant difference. The small number ( n = 8) of post‐transplant CD8 + clones showed decreased production of IL‐3, IL‐4 and IL‐6 compared with control clones, but normal secretion of GM‐CSF. Neither CD4 + nor CD8 + T‐cell clones secreted interleukin‐7 (IL‐7).