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Preferential Rearrangements of the VγI Subgroup of the γ‐Chain of the T‐Cell Antigen Receptor to Jγ2Cγ2 Gene Segments in Peripheral Blood Lymphocyte Transcripts from Normal Donors
Author(s) -
MATHIOUDAKIS G.,
CHEN P.F.,
LI Y.D.,
CHERNAJOVSKY Y.,
PLATSOUCAS C. D.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01690.x
Subject(s) - t cell receptor , microbiology and biotechnology , gene , biology , gene rearrangement , complementary dna , polymerase chain reaction , t cell , genetics , immune system
To investigate whether there are preferential VJC gene rearrangements of the γ‐chain of the human T‐cell antigen receptor (TCR), we amplified and sequenced γ‐chain TCR transcripts from peripheral blood lymphocytes from adult normal donors. cDNA was synthesized from total RNA and amplified by the polymerase chain reaction (PCR) using 5’primers specific for either the VγI or the VγII subgroups of the γ‐chain of the TCR. The amplified cDNAs were then cloned and sequenced. The majority (approximately 83%) of the cDNAs employing V‐I subgroup gene segments rearranged to Jγ2 (Jγ2.1 or Jγ2.3) Cγ2 gene segments. This was in contrast to the predominant rearrangement of the VγII subgroup (Vγ9) to JγL2CγL The remaining 13% of the cDNAs employing VγI subgroup gene segments rearranged to JγLlCγl or JγL3Cγl. There was significant N diversity as well as imprecise joining at the VJ junction, γδ5 TCR utilizing the Cγ l gene segment are disulfide‐linked, whereas those utilizing the Cγ2 gene segment are non‐disulfide‐linked. These results demonstrate that peripheral blood γ‐chain transcripts exhibit preferential rearrangements of VγI subgroup gene segments to Jγ2(2.1,2.3)Cγ2 gene segments. By contrast, VγII subgroup (Vγ9) transcripts exhibit rearrangements to Jγ1.2Cγ1.