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T‐Cell Receptor Alpha Haplotype influences Vα Epitope Expression on both Cortisone‐Resistant Thymocytes and Lymph Node T Cells
Author(s) -
BOGEN B.,
GLEDITSCH L.,
TEIG A.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01685.x
Subject(s) - epitope , haplotype , microbiology and biotechnology , biology , monoclonal antibody , t cell , allele , antibody , genetics , gene , immune system
We have previously described a mouse strain congenic for the T‐cell receptor alpha chain locus ( Tcra ). The strain, B10.D2.C‐ Tcra a /Bo, carries the Tcra a haplotype of BALB/c instead of the Tcra a haplotype normally found in B10.D2. By comparing B10.D2.C‐ Tcra a and B10.D2 mice, we now show that the Tcra haplotype influences the frequencies of Vα8 epitope expression on T cells detected by the novel B21.14 monoclonal antibody (MoAb). This finding agrees well with our previous observation that Tcra haplotype influences the frequencies of expression of two other Vα8 epitopes, detected by the KT50 and KT65 MoAbs. The specificities of the three Vα8‐specific MoAbs were compared by scatter diagram analysis of staining frequencies obtained in individual mice. The B21.14 and KT50 MoAbs appear to have very similar specificities; these two MoAbs stain slightly more αβ T cells than the KT65 MoAb. Investigations with another novel MoAb, the Vα2‐specific B20.1, revealed that the Tcra haplotype also influences the frequency of Vα2 epitope expression. The effect of the Tcra haplotype on Vα2 epitope expression was evident not only among lymph node T cells but also among mature, cortisone‐resistant thymocytes. Thus, the influence of the Tcra haplotype is imprinted already in the thymus. The most likely explanations for Tcra haplotype dependency of Vα epitope expression are differing numbers of Vα‐gene segment subfamily members and/or allelic polymorphism. The significance of Tcra haplotype‐dependent Vα epitope expression is discussed.