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Modulation and High Frequency Expression of Autoantibody‐Associated Cross‐Reactive Idiotypes linked to the V h I Subgroup in CD5‐Expressing B Lymphocytes from Patients with Chronic Lymphocytic Leukaemia (B‐CLL)
Author(s) -
SHOKRI F.,
MAGEED R. A.,
RICHARDSON P.,
JEFFERIS R.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01682.x
Subject(s) - cd5 , immunoglobulin light chain , germline , antibody , b cell , chronic lymphocytic leukemia , epitope , cd19 , microbiology and biotechnology , biology , immunology , lymphoproliferative disorders , autoantibody , idiotype , monoclonal antibody , gene , medicine , lymphoma , leukemia , genetics
Leukaemic B cells from patients with chronic lymphocytic leukaemia (B‐CLL) are known to express the pan T‐cell marker CD5 and a restricted set of immunoglobulin (Ig) variable region heavy (V h ) and light (V L ) chains encoded by germline or minimally mutated germline genes. We have studied surface expression of certain V H and V K gene products on peripheral blood B lymphocytes from 23 patients with B‐CLL, using a panel of monoclonal antibodies (MoAbs) recognizing germline encoded cross‐reactive idiotypes (CRI) associated with V H I (G6, G8), V H III (B6, D12), V K IIIb (17–109) and an epitope linked to the V K III light chain subgroup (C7). While only 1.7–3.2% of peripheral blood B lymphocytes from normal individuals expressed the V H I‐associated CRI (V h I‐CRI), these CRI were expressed on virtually all the leukaemic B cells from 17–22% of the CLL patients. The V H III‐associated CRI (V H III‐CRI), however, were found in 8.5–13% of the CLL B cells. Fifty per cent of the IgMK‐expressing CLL cells (7/14) expressed the V R III light chain subgroup of which only one expressed the V K IIIb‐associated CRI (V K IIIb‐CRI), 17–109. The anti‐V H I‐associated CRI antibodies were used to study their regulatory effect on in vitro Ig synthesis by the leukaemic cells. A significant suppression of spontaneous and mitogen‐driven Ig production was observed in all cases studied. These results demonstrate an over‐expression of V H I and V K III gene products in B‐CLL and suggest that B cells expressing these CRI are particularly susceptible to lymphoproliferative stimuli. The anti‐CRI antibodies can be used to modulate Ig production by the leukaemic cells and may be of potential value for selective immunotherapy.