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Anti‐Tumour Activity of Idiotype‐Specific, MHC‐Restricted Th1 and Th2 Clones In Vitro and In Vivo
Author(s) -
LAURITZSEN G. F.,
WEISS S.,
BOGEN B.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01668.x
Subject(s) - cytotoxic t cell , biology , microbiology and biotechnology , lymphoma , clone (java method) , transfection , idiotype , in vitro , t cell , major histocompatibility complex , cell culture , mhc class i , immunology , antibody , antigen , monoclonal antibody , immune system , gene , genetics
Idiotypes (Id) can serve as individual markers on B cells; therefore, cytotoxic Id‐specific T cells may play a significant role in immunological surveillance of Id + B‐cell tumours. We have investigated the anti tumour activity of CD4 + BALB/c Thl and Th2 clones which recognize a processed Id of the syngeneic λ2 315 L chain in the context of the class II MHC molecule I‐E d . Id‐specific T cells and A 20/46 B lymphoma cells transfected with the λ2 315 gene were injected s.c. into the same site of BALB/c mice (Winn assay). The results show that both Th l and Th2 clones can protect against tumour development. The protection was Id‐specific because T cells did not influence tumour development by an A20/46 B lymphoma cell line transfected with the pSV2neo expression vector alone. In vitro studies showed that the Th1 clones were cytotoxic to λ2 315 ‐transfected B lymphoma cells; by contrast, the Th2 clone was not cytotoxic in 5l Cr‐release assay even though the Th2 cells inhibited the growth of λ2 315 B lymphoma cells. The anti lympboma properties of both the Thl and Th2 clones appear to involve as yet undefined cytotoxic and growth inhibiting molecules.