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The Priming of Cytotoxic T‐Cell Precursors is Strictly Helper T Cell‐Dependent
Author(s) -
LANGMAN Y. LIN. R.,
COHN M.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03262.x
Subject(s) - cytotoxic t cell , priming (agriculture) , t cell , t helper cell , immunology , biology , microbiology and biotechnology , chemistry , immune system , genetics , in vitro , botany , germination
Allogeneic chimaeras that utilize C. B. 17 SCID mice (H‐2 d ) as recipients of MHC mismatched bone marrow from C57B1/6(H‐2nd) or SJL/J (H‐2 d ) mice have been used to provide experimental evidence demonstrating the necessity for a direct interaction between an effector T helper and the cytotoxic T‐cell precursor in order to generate cytotoxic effector T cells specific for the minor histocompatibility (H) antigens of DBA/2 (H) mice. No effect of helpers specific for antigen processed or not‐presented on antigen‐presenting cells could be observed. Allo‐chimaeras that contain T cells hearing H‐2 d , and which are restricted to H‐2 d , make a cytotoxic T‐cell response to minor H antigens which is H‐2 d restricted if, and only cloned anti‐H‐2 d effector T helpers are present during the vivo printing step. Cloned anti‐H‐2 d effector helpers, which are without effect in the allo‐chimaeras, do provide a strong helper activity when tested in normal H‐ 2d mice. These findings cannot reconciled with a strict single recognitive model of restrictive antigen recognition, but they are consistent with a dual recognitive model, which incorporates many of the features of the single recognitive model.