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Presence of CD 16 on Endothelial Cells in Heart Transplant Rejection: an Immunohistochemical Study
Author(s) -
TUIJNMAN W. B.,
WIJNGAARD P. L. J.,
WICHEN D.,
WINKEL J. G. J.,
CAPEL P. J. A.,
SCHUURMAN H.J.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03256.x
Subject(s) - immunohistochemistry , antibody , pathology , immunofluorescence , von willebrand factor , endothelium , cd16 , biology , transplantation , lymphatic system , flow cytometry , heart transplantation , endothelial stem cell , microbiology and biotechnology , immunology , antigen , medicine , cd3 , platelet , endocrinology , cd8 , in vitro , biochemistry
We studied the distribution of Fcγ RIII (CD16) in human lymphoid and non‐lymphoid tissue by immunohistochemistry and two‐colour immunofluorescence. In all tissues except stomach, skin, muscle and heart, infiltrating cells were stained. In tonsil and thymus, the CDI6 antibody CLBgran I labelled some cells with high endothelial morphology in T‐cell areas. Two‐colour fluorescence combination of CD 16 and antibody to Von Willebrand factor confirmed the endothelial nature of the CLBgran l‐positive cells. FcγRIII expression was also demonstrated by antibody CLBgran 11, which detects a product of the FcγRIII‐B gene of only the NAI haplotype. A series of 95 endomyocardial biopsies (FMB) taken after heart transplantation was evaluated for FcγRIII expression. In 18 eases FcγRIII was unambiguously detected on endothelial cells by CD16 antibody CLBgranI. There was a significant correlation between capillary CD16 expression and transplant rejection. We conclude that FcγR expression on endothelium represents endothelial activation found in immunopathological and inflammatory conditions.