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T‐Cell Receptor Identification of an Oligodendrocyte‐Specific Autoreactive Cytotoxic T‐Cell Clone Without Self Restriction
Author(s) -
JEWTOUKOFF V.,
AMZAZI S.,
LEBAR R.,
BACH M. A.,
MARCHE P. N.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03152.x
Subject(s) - myelin oligodendrocyte glycoprotein , t cell receptor , cytotoxic t cell , clone (java method) , biology , t cell , proteolipid protein 1 , myelin basic protein , microbiology and biotechnology , alpha chain , experimental autoimmune encephalomyelitis , beta (programming language) , immunology , myelin , multiple sclerosis , receptor , gene , central nervous system , genetics , immune system , neuroscience , in vitro , computer science , programming language
In addition to myelin basic protein (MBP) and proteolipid protein (PLP), oligodendrocyte (Od) membrane autoantigens, such as the glycoprotein M2/MOG, could participate in the pathogenesis of autoimmune demyelinating diseases of the central nervous system (CNS), such us experimental allergie encephalomyelitis (EAE) or multiple sclerosis (MS). We have described an Od‐specific autoreactive and cytotoxic T‐cell clone, named C2, which recognized M2/MOG without conventional MHC restriction. In order to analyse the Od/C2 interaction, we determined the α/β T‐cell receptor (TCR) variable region usages and structures of C2. Monoclonal antibody stainings of C2 and nucleotide sequences show that the α chain is composed of a Vα5 and a Jα identical to Jα18BBM 142 gene segments, and that the TCR β chain is composed of Vβ17a, Dβ2.1 and Jβ2.2 gene segments indicatmg that C2 used a conventional α/β TCR for M2/MOG recognition.