Premium
Primary Stimulation of CD4 + Cells in the Presence of IL‐4 or IFN‐γ Alters the Frequencies of Cytokine‐Producing Cells at Restimulation
Author(s) -
CARDELL S.,
SANDER B.,
MÖLLER G.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03138.x
Subject(s) - lymphokine , cytokine , microbiology and biotechnology , cd8 , antigen presenting cell , t cell , biology , cytotoxic t cell , interleukin 21 , interleukin 4 , interleukin 12 , interleukin 3 , interleukin 2 , immunology , chemistry , antigen , in vitro , immune system , biochemistry
The induction of specific effector functions in naive T cells may be directed by accessory signals during activation. These could be elicited through binding to cell surface molecules or through factors secreted by antigen‐presenting cells or other simultaneously activated cells. We have investigated the influence of CD8 + cells and of exogenousiy added cytokines (interleukin (IL)‐2, IL‐4 and interferon (IFN)‐γ) on the cylokine production in splenic CD4 + T cells. IL‐2, IL‐4, IL‐5 and IFN‐γ production in CD4 + cells was measured at the single cell level during primary mitogen stimulation in vitro in the presence or absence of factors or CD8 + cells. On day 5 the cells were restimulated with mitogen alone and analysed to evaluate the short‐term development of cytokine‐producing cells in such cultures. Preactivation in the presence of either exogenous IL‐4 or IFN‐γ led to an increased production of IL‐4 and IFN‐γ respectively at restimtilation, and the effects of both IL‐4 and IFN‐γ were augmented by IL‐2. After preactivation in the presence of IL‐2 and IL‐4, every third CD4 + cell could be induced to produce IL‐4. Exogenous IL‐4 or IFN‐γ further decreased each other's production. Depletion of CD8 + cells before activation resulted in a slight increase of IL‐4‐producing cells, indicating that simultaneous activation of CD8 + cells will influence lymphokine production in CD4 + cells. The results suggest that the pattern of lymphokines induced in naive cells may be influenced by factors secreted by preactivated CD4 + and CD8 + cells, and that naive cells are preferentially ‘recruited’ to produce similar cytokines.