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Isoelectrophoretic Characterization of Protein Antigens Present in Mycobacterial Culture Filtrates and Recognized by Monoclonal Antibodies Directed Against the Mycobacterium bovis BCG Antigen 85 Complex
Author(s) -
DROWART A.,
BRUYN J.,
HUYGEN K.,
DAMIANI G.,
GODFREY H. P.,
STELANDRE M.,
YERNAULT J.C.,
VOOREN J.P.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03130.x
Subject(s) - mycobacterium bovis , monoclonal antibody , antigen , microbiology and biotechnology , biology , epitope , tuberculosis , mycobacterium tuberculosis , mycobacterium smegmatis , western blot , isoelectric focusing , antibody , virology , mycobacterium , immunology , bacteria , medicine , biochemistry , genetics , pathology , gene , enzyme
Nine monoclonal antibodies (MoAbs) were produced againsi Mycobacterium bovis BCG aniigen 85 complex. Using isoelectric focusing combined with Western (immunoblot) blot analysis, antigenically related proteins could be identified in culture filtrates from M. tuberculosis, M. bovis. M. kansasii, M. aviumi, M. xenopi, M. gordonde, M. fortuitum, M. phlei M.smegmatis , Most of the MoAbs were found to be broadly cross‐reactive between the various mycobacterial species, albeit some minor differences were observed. These MoAbs reacted generally, in each species, with different components. One MoAb (VIDl‐14) was found to be specifically directed only against antigen 85B from M. bovis. M. tuberculosis and M. kunsasii .