Premium
Human Blood Dendritic Cells Exhibit a Distinct T‐Cell‐Stimulating Mechanism and Differentiation Pattern
Author(s) -
XU H.,
FRIEDRICHS U.,
GIESELER R. K. H.,
RUPPERT J.,
OCKLIND G.,
PETERS J. H.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03129.x
Subject(s) - t cell , cd14 , biology , microbiology and biotechnology , monoclonal antibody , antigen , antigen presenting cell , cd40 , mhc class ii , cytotoxic t cell , immunology , major histocompatibility complex , antibody , flow cytometry , immune system , in vitro , biochemistry
In this study, the mechanisms underlying stimulation of T‐cell proliferation by human blood dendritic cells (BDC) and their differentiation have been defined with a panel of monoclonal antibodies (MoAbs)‐ It was found that the MoAbs against LFA‐I (CDlla), CD11c. LFA‐3 (CD58), ICAM‐1 (CD54) or HLA‐DR could significantly suppress T‐cell proliferation in an allogeneic mixed lymphocyte reaction ( P <0.05), while being unable to inhibit clustering of BDC with T cells. Addition of anti‐CD18 or CD45 MoAbs increased the size of clusters after 18 h of culture, but had no effect on the proliferation of T cells (P<0.05). The suppressive effect of the MoAbs may be viewed not as an inhibition of contact between BDC and T cells, but rather as a blocking of co‐stimulatory signals for T‐cell activation, which are mediated by interaction of the adhesion molecules. After depleting the BDC preparations of monocytes. we used a double staining in FACS analysis to demonstrate that BDC do not express specific T (CD3), B (CD20 and CD2l)and myeloid cell markers (CDl1b, CDl3 and CD14), but abundant class II antigens. This pattern remained unaltered after 8 days of culture in the presence of 100 U/ml GM‐CSF, although a threefold increase of HLA‐DQ and ICAM‐1 molecules on the cultured cells was observed.