Premium
Involvement ofthe Avian μ Heavy Chain in Recolonization ofthe Bursa of Fabricius
Author(s) -
PALOJOKI E.,
LASSILA O.,
JALKANEN S.,
TOIVANEN P.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03097.x
Subject(s) - bursa of fabricius , polyclonal antibodies , epitope , antibody , monoclonal antibody , incubation , biology , cyclophosphamide , microbiology and biotechnology , virology , immunology , genetics , biochemistry , virus , chemotherapy
In Ihe chicken, the B eells develop in a specialized organ, the bursa of Fabricius. Earlier it was shown that neonatal bursal cells treated with polyclonal anti‐chicken immunoglobuiin antibodies are not able to recolonize tho bursa when transferred into cyclophosphamide‐treated chicks. In this study, 4‐day‐old bursal eells were treated with different polyclonal and monoclonal antiimmunoglobulin antibodies and transferred into 4‐day‐old eyelophosphamide‐treated chickens. Two monoclonal anti‐chicken IgM antibodies. CVI‐59.7 and 21‐2B2. recognizing distinct epitopes of the μ heavy chain, were inhibitory. Incubation of cells with 21‐2B2 antibody caused about 9O% inhibition of bursal recolonization. After incubation with CVI‐59.7 antibody ihe inhibition was 50%, The high inhibition by 21‐2B2 antibody was also seen when F(ab') 2 fragments of the antibody were used. These results suggest that the entry of the cells needed for bursal reeolonization is inhibited almost totally by 21‐2B2 antibody, or that this antibody blocks further proliferation of the cells in bursal follicles. In eonelusion, we have shown that a μ heavy chain epitope is intimately involved in the recolonization of bursal follicles, and distinct epitopes of the (I heavy chain are not equally important in this proeess.