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Enhancement of the Antibody Response to Protein Antigens by Specific IgG under Different Experimental Conditions
Author(s) -
WIERSMA E. J.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb03091.x
Subject(s) - antigen , antibody , immunology , antibody response , biology
Specific stimulation of the antibody response to protein iintigcns by IgG antibodies was studied in vivo‐The response to TNP‐coupled keyhole limpet haemocyanin (KLH‐TNP) was enhanced by a TNP‐spcciftc IgG monoclonal antibody, 7B4, as measured both in ELISA (enzyme‐linked immunosorbent assay) and by ELISPOT (enzyme‐linked immunospot) method. Enhancement was seen when using both high and low doses of antigen and antibody. The antibody response to TNP‐coupled bovine serum albumin (BSA‐TNP), bul not to TNP‐couplcd ovalbumin (OA‐TNP), tetanus toxoid (TT‐TNP) or diphtheria toxoid (DT‐TNP). was also etficiently augmented by 7B4, Enhancement was seen when using both high and low coupling ratio of TNP to KLH, The fact that IgG‐mediiited enhancement is seen under many ditVerent experimental conditions suggests that it is a physiologically relevant phenomenon. In order lo enhance the response to KLH‐TNP and BSA‐TNP, 7B4 had lo be injected while antigen was circulating in blood. This supports that igG‐mediated stimulation acts by concentrating circulating antigen into lymphoid centres.