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Cells of Cerebrospinal Fluid of Multiple Sclerosis Patients Secrete Antibodies to Myelin Basic Protein In Vitro
Author(s) -
CASH E.,
WEERTH S.,
VOLTZ R.,
KORNHUBER M.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02977.x
Subject(s) - polyclonal antibodies , pathogenesis , peripheral blood mononuclear cell , cerebrospinal fluid , myelin basic protein , multiple sclerosis , antibody , western blot , immunology , in vitro , pokeweed mitogen , myelin , medicine , biology , pathology , central nervous system , biochemistry , gene
To characterize the role of B lymphocytes in the pathogenesis of Multiple Sclerosis (MS), we have isolated mononuclear cells from cerebrospinal fluid (CSF) and stimulated them with a polyclonal B‐cell mitogen (pokeweed mitogen). This study has been done with MS patients selected for the occurrence of an acute attack in the course of the disease and with patients hospitalized for other neurological diseases. Five of the 11 MS patients had B lymphocytes producing in vitro antibodies (Abs) directed against purified human myelin basic protein (hMBP), as revealed by Western blot analysis. None of the 20 patients with other neurological diseases showed such a reactivity. The produced Abs recognized only 1 or 2 hMBP peptides without dominance for a certain peptide. This result emphasizes the presence of B cells producing Abs against MBP in CSF of MS patients and shows the interest of studying mononuclear cells of CSF as a good marker of the pathogenesis