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The Effect of Calcium Mobilization on LPS‐Induced IL‐1β Production Depends on the Differentiation Stage of the Monocytes/Macrophages
Author(s) -
HURME M.,
VIHERLUOTO J.,
NORDSTRÖM T.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02966.x
Subject(s) - calcium , ionophore , monocyte , intracellular , calcium in biology , cell culture , chemistry , microbiology and biotechnology , macrophage , cellular differentiation , cell type , endocrinology , medicine , cell , biology , immunology , biochemistry , in vitro , genetics , gene
The role of elevated intracellular Calcium concentration [Ca 2+ ] i in the LPS‐induced activation of interleukin‐1β (IL‐1β) production was examined in cells representing different stages of myeloid differentiation (undifferentiated monocytic leukaemia cell line THP‐1, THP‐1 cells induced to adherent, macrophage‐like cells by phorbol ester treatment and normal peripheral blood‐derived adherent monocytes). LPS did not elevate the [Ca 2+ ] 1 , as measured by the Fura‐2 fluorescence technique. When these cells were stimulated with LPS in the presence of the calcium ionophore A23187, a clear increase in the IL‐ 1β protein production was observed in the undifferentiated THP‐1 cells but not in the more differentiated cell types. This ionophore‐induced increase was also seen in the IL‐1β mRNA levels. Thus these data confirm the previous findings demonstrating that elevation of (Ca‐ 2+ ] 1 is not involved in the LPS‐dependent signal transmission. However, the LPS‐induced signals are greatly potentiated by the elevated [Ca 2+ ] i , but only in undifferentiated monocytic cells.