Premium
Expression of CD11b (Leu15) Antigen on CD3 + , CD8 + , CD16 + Peripheral Lymphocytes, Estimation of CD3 + 8 + 11b + and CD3 + 4 ‐ 8 ‐ 11b ‐ T‐Cell Subsets using a Single Laser Flow Cytometer
Author(s) -
GANE P.,
FAIN O.,
MANSOUR I.,
ROQUIN H.,
ROUGER P.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02953.x
Subject(s) - cd8 , cd16 , cytotoxic t cell , cd3 , antigen , biology , microbiology and biotechnology , t lymphocyte , epitope , immunology , in vitro , biochemistry
CD11b (Leu15) epitope is expressed on 20‐30% of peripheral blood lymphocytes, including CD16 + large granular lytnphocytes and CD8 + cells. This study confirms that 30% of CD8 + lymphocytes and virtually all CD16 + NK cells from healthy subjects express this determinant. In parallel, our data show that various proportions of CD3 + 4‐8‐, TCR‐δ cytotoxic T lymphoeytes and occasionally CD4 + lymphocytes subsets could also express this epitope. The CD8 + 11b + phenotype is associated with suppression of T‐cell proliferative response and has been extensively used to characterize suppressor T lymphocytes. Since about 25% of CD8 lymphocytes are non‐T (CD3) and express the CD16 NK antigen (CD8 + 16 + 3 ‐), the expression of CD11b was also studied on CD8 + 3 + T‐cell and CD8 + 16 + NK‐cell subsets. To this end, we developed three methods using a flow cytometer equipped with a single laser and two fluorescence detectors. Results showed that T CD8 + 3 + 11b + and NK CD8 + 16 + 11b + lymphocytes account for 3O% and 70% of CD8 + 11b + cells respectively. Consequently, the CD8 + 3 + 11b + phenotype would be more specific for suppressor T lymphocytes than the total CD8 + 11b + phenotype which includes high proportions of CD16 + NK cells.