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Effect of rIFN‐γ on Antibody‐Mediated Cytotoxicity via Human Monocyte IgG Fc Receptor II (CD32)
Author(s) -
SCHIE R. C. A. A.,
VERSTRATEN H. G. G.,
TAX W. J. M.,
BERKMORTEL F. W. P. J.,
WINKEL J. G. J.,
MULDER P. H. M.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02952.x
Subject(s) - antibody dependent cell mediated cytotoxicity , fc receptor , receptor , monocyte , antibody , cytotoxicity , microbiology and biotechnology , cell culture , immunoglobulin fc fragments , recombinant dna , receptor expression , fragment crystallizable region , chemistry , immunoglobulin g , immunology , in vitro , biology , monoclonal antibody , biochemistry , gene , genetics
Human monocytes and macrophages express an isoform of IgG Fe receptor II (FcγRII), FcγRII. Two allotypic variants of this receptor could be distinguished with respect to their ability to bind murine (m)IgG1 complexes either strongly or weakly, defined as high‐responder (HR) and low‐responder (LR). respectively. We investigated the effect of recombinant (r)IFN‐γ on the ability of freshly isolated monocytes, and those cultured for 40 h and 9 days, to mediate antibody‐dependent cell‐mediated cytotoxicity (ADCC), Using human erythrocytes (E) sensitized with mlgGl as target cells, FcγRII was studied selectively. Cells which had been cultured for 40 h exhibit a significantly decreased FcγRII expression, and FcγRll‐mediated ADCC activity as compared with freshly isolated monocytes. Co‐culture with rlFN‐γ (40 h) reversed this decrease. Short‐term rlFN‐γ‐cultured cells, and fresh cells express similar numbers of FcγRII, and exhibit comparable FcγRII‐mediated ADCC activity. Phagocytic activity was not affected. Prolonged culture of monocytes for 9 days, co‐cultured with rlFN‐γ either from day 0 or from day 7, did not affect expression or functional activity of FcγRII. Furthermore, the effects were observed in both HR and LR individuals. Our results show that rlFN‐γ has strong effects on FcγRII‐mediated responses specifically during the early stages of monocyte maturation, most likely by affecting receptor expression levels.