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Role of ELAM‐1 in Adhesion of Monocytes to Activated Human Endothelial Cells
Author(s) -
LEEUWENBERG J. F. M.,
JEUNHOMME T. M. A. A.,
BUURMAN W. A.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02866.x
Subject(s) - adhesion , cell adhesion molecule , cell adhesion , monocyte , cd18 , microbiology and biotechnology , e selectin , chemistry , biology , immunology , flow cytometry , integrin alpha m , organic chemistry
The role of ELAM‐1 in the adhesion of monocytes to HUVEC, activated for 4 h with TNF, was studied using MoAb ENA2 directed against ELAM‐1. In a standard adhesion assay at 37 C, F(ab') 2 fragments of f ENA2 did not, or weakly inhibited adhesion. When metabolic activity of the monocytes was reduced by (i)fixing the monocytes, (ii) performing the adhesion assay at 4°C, and (iii) combining the forementioned conditions, the adhesion of the monocytes was strongly blocked by ENA2 and less effective or not by MoAb IB4 anti‐CD18. The pattern of adhesion of monocytes to HUVEC, activated with TNF assessed at 4°C. paralleled ELAM‐1 expression on the endothelial cells. Maximal inhibitory effect of ENA2 on adhesion was shown 5 h after activation of HUVEC, at which ELAM‐1 expression was also maximal. Adhesion assessed at 37°C remained enhanced for at least 24 h, whereas the inhibitory effect of ENA2 followed ELAM‐1 expression. Specific involvement of ELAM‐1 was also confirmed using ELAM‐1 transfected COS cells. These results indicated that monocytes express a counter structure for ELAM‐1 and that this counter structure is involved in adhesion.