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Prolonged Elevation of Intracellular Cyclic AMP Activates Interleukin‐1 Production in Human Peripheral Blood Monocytes
Author(s) -
SERKKOLA E.,
HURME M.,
PALKAMA T.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb02851.x
Subject(s) - peripheral blood , intracellular , peripheral , interleukin , elevation (ballistics) , medicine , immunology , biology , cytokine , microbiology and biotechnology , physics , astronomy
The capability of elevated intracellular cyclic AM P concentration to activate IL‐l gene expression and protein production was examined in human peripheral blood monocytes. In accordance with previous studies it was observed that the transiently elevated cyclic AMP (induced either with prostaglandin E2 or with the direct adenylate cyclase activator, forskolin) was not a sufficient signal to activate IL‐l production. However, if the degradation of cyclic AMP was inhibited with isobutyl‐methyl‐xanthine (IBMX). IL‐l production was strongly activated. This prostaglandin E2 plus IBMX effect could also be mimicked with high concentrations of the cell permeant structural cyclic AMP analogue, dibutyryl cyclic AMP. The cyclic AMP4nduced IL‐l production differed in some aspects from the bacterial lipopolysaccharide‐induced IL‐l production: (1) the kinetics of both IL‐l gene expression and protein production was much slower: (2) the IL‐1β gene expression was superinducible by inhibiting the protein synthesis with cycloheximide. Thus these data suggest that prolonged elevation of cyclic AMP is alone a sufficient signal to activate IL‐l production.