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Generation of Lymphokine‐Activated Killer Cells by Adherent LGL Phenotype Cells and Non‐Adherent T Lymphocytes
Author(s) -
OUESLATI R.,
MAALEJ M.,
CHOUIKHA M.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb01648.x
Subject(s) - lymphokine activated killer cell , lymphokine , population , phenotype , k562 cells , immunology , microbiology and biotechnology , biology , lymphocyte , cell culture , interleukin 2 , incubation , cytokine , chemistry , t cell , medicine , interleukin 21 , antigen , immune system , biochemistry , leukemia , genetics , environmental health , gene
In this work we separated human blood lymphocytes (PBL) in two populations (A‐LAK and NALAK cells) by the adherence plastic method. A maximum adherence of cells was obtained after 2 days of PBL incubation in LAK medium containing 500 U/ml rIL‐2. The A‐LAK cells had LGL phenotype but 40% of them had a macrophage phenotype marker and less than 20% weakly expressed a T‐cell marker. This population, when reincubated in culture, produced an increasing titre of interferon. At the same time, a significant NK activity against K562 target cells was measured just after enrichment; these enriched adherent cells also developed an increased LAK activity against DAUDI cell lines, ninefold more at 6 days than when assayed just after enrichment. In contrast, 75% of the NA‐LAK enriched cells expressed T‐cell marker; these produced two‐ to threefold less interferon than A‐LAK cells at all time‐points. The NA‐LAK lymphocytes enhanced principally LAK activity measured by 70% lysis against DAUDI target cells tested at 6 days of culture. Further studies are in progress to determine the nature of the effector cells that mediated LAK activity.