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Immunoblot Analysis of Sera from Ethiopian Cutaneous Leishmaniasis by Antibody Class
Author(s) -
MENGISTU G.,
AKUFFO H.,
FEHNIGER T. E.
Publication year - 1992
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1992.tb01639.x
Subject(s) - antibody , antigen , immunology , leishmaniasis , cutaneous leishmaniasis , immunoglobulin e , leishmania , virology , medicine , biology , parasite hosting , world wide web , computer science
We have determined the specificity of the classes of anti‐leishmanial antibodies which are delectable in serum from patients with active cutaneous leishmaniasis. On immunoblots, differences exist in the patterns of antigen recognition by IgG, IgM, IgA and IgE antibodies present in localized cutaneous leishmaniasis (LCL) and diffuse cutaneous leishmaniasis (DCL) patient serum. Each class of antibody showed differing patterns of banding to the various molecular species of parasite antigens. There was significant variation in the specificities of the IgG antibody reactivities between individual patients. The patterns of IgM binding were generally homogeneous and restricted to antigens with M r 40 kDa. The only class of anti‐ Leishmainia antibodies which showed shared patterns of common antigen recognition by all of the patients studied were IgA antibodies. The reactivities of IgE antibodies encompassed two antigens of M r 36 and 46‐48 kDa which were not recognized by any of the other isotypes. Such antibody class associated reactivity may be useful in the design of serodiagnostic assays for the detection of Leishmania infection or other infectious agents. Gennene Mengistu, Department of Infectious Diseases, K. Karolinska Institute, Huddinge Hospital, 14186 Huddinge, Sweden