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A Rapid Microtitration Plate Assay for Non‐Specific Esterase
Author(s) -
SONNE O.,
PEDERSEN K.,
KUDAHL K.,
FISKER S.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb03754.x
Subject(s) - esterase , homogeneous , microbiology and biotechnology , chemistry , colorimetry , chromatography , biochemistry , biology , enzyme , mathematics , combinatorics
A simple and convenient colorimetric assay for the quantification of non‐specific esterase is described. The assay takes advantage of the microtitration plate format, and the high capacity of repetitive dispensers and the microplate reader for this format. The assay is well suited for the quantification changes in the esterase activity in homogeneous cell populations like monocytes maturing into macrophages.