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Allorecognition of HLA DR2 and DR5 Molecules by V‐Beta‐8‐Positive T‐Cell Clones
Author(s) -
WILSON K. E.,
BALL E.,
STASNY P.,
CAPRA J. D.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb03743.x
Subject(s) - allorecognition , peripheral blood mononuclear cell , microbiology and biotechnology , t cell , human leukocyte antigen , biology , monoclonal antibody , antigen , major histocompatibility complex , immunology , beta (programming language) , t lymphocyte , antibody , genetics , in vitro , immune system , computer science , programming language
V‐beta‐8‐positive T cells were isolated from human peripheral blood lymphocytes using a monoclonal antibody specific for the Vβ8 family. Alloreactive T‐cell lines were generated by stimulation with mononuclear cells from individuals homozygous for HLA DRI‐DR9. Cloned Vβ8‐positive T cells were then assayed for alloreactivity based on a proliferative assay using irradiated B‐cell lines. Vβ8‐positive T‐cell clones alloreactive to DR2 and DR5 molecules were chosen for further study based on the association of these MHC antigens with autoimmune disease. DNA sequence analysis confirmed the use of the Vβ8 gene family as well as providing information on the use of the V, D, J and N segments in these alloreactive T‐cell clones.