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Different Activation Signals are Required for the Expression of Interleukin‐1 α and β Genes in Human Monocytes
Author(s) -
HURME M.,
SERKKOLA E.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb02545.x
Subject(s) - protein kinase c , activator (genetics) , microbiology and biotechnology , messenger rna , northern blot , lipopolysaccharide , gene expression , phorbol , gene , protein kinase a , stimulation , tetradecanoylphorbol acetate , interleukin , biology , kinase , chemistry , cytokine , biochemistry , immunology , endocrinology
The production and mRNA expression of IL‐1α and IL‐1β by human monocytes was examined after two different stimuli, a protein kinase C (PKC) activator phorbol myristate acetate (PMA) and bacterial lipopolysaccharide (LPS). LPS induced production of high levels of both IL‐la and IL‐1β protein (quantitated with type‐specific ELISA assays), while after PMA stimulation only IL‐1/β protein could be detected. The IL‐1α and IL‐1/β mRNA levels quantitated by Northern blotting were in line with the respective protein levels and nuclear run off analysis revealed that PMA did not activate the IL‐la transcription. The production of the IL‐1α and IL‐1/β protein as well as the mRNA expression could be inhibited with protein kinase inhibitor H7, but not with HA 1004, indicating that PKC activation is essential for the activation of these genes. Thus these data indicate that PKC activation alone is sufficient for the induction of the IL‐1/β gene, but some additional signals (provided by LPS) are required for the activation of the IL‐1α gene.