Premium
C5 Does Not Play a Major Role in the Immune Response of Mice to SRBC In Vivo
Author(s) -
BERG C. W.,
HORSSEN P. J.,
HOFHUIS K. M. A.,
AERTS P. C.,
DIJK H.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb02534.x
Subject(s) - in vivo , immune system , congenic , in vitro , antigen , immunology , biology , ratón , biochemistry , gene , genetics
The role of complement component C 5 in the immune response of mice to sheep red blood cells (SRBC) was investigated, Congenic C5‐suffcieal and C 5‐deficient B10.D2 mice Lind genetically CS‐deficient DBA/2 mice. as such or supplemented with C5‐suficient serum, were used as experimental animals. C5‐substitution of the C5‐deficient mice resulted m measurable CS levels for days. The functional half‐life of C? in C5‐deficienl DBA 2 mice was about 21 h. No significant differences between the IgM‐responses of C5bearing and naive C5‐delicient animals were observed. This suggests t ha C5 does not play a major role in the primary humoral immune response of mice in vivo, although C5 seems to do so in in vitro experiments, even with the same antigen. Antigen‐induced C5‐production by C5‐defieient mice as one of the explanations of the in vitro, in vivo discrepancy could not be confirmed experimentally.