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‘E‐Boxes’ as Promoter Elements in B Cell Lines and Untransformed B Lymphocytes
Author(s) -
HÖGBOM E.,
MAGNUSSON A.C.,
LEANDERSON T.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb02533.x
Subject(s) - tata box , microbiology and biotechnology , electrophoretic mobility shift assay , enhancer , biology , b cell , intron , stimulation , cell culture , transcription (linguistics) , cell , dna , promoter , chemistry , transcription factor , gene , antibody , gene expression , genetics , linguistics , neuroscience , philosophy
The penta‐deca (pd) promoter element from the SP6 K promoter and the μE2 box from the Ig heavy chain intron enhancer were analysed in an electrophoretic mobility shift assay (EMSA), utilising cell extracts from total mouse splenic B cells before and after stimulation with lipopolysaccharide. With both probes a changed EMSA pattern was observed after stimulation of the cells, where higher molecular weight DNA/protein complexes became dominant. When the pd and μE2 sequence elements were cloned in front of a TATA box and analysed for their transcriptional promoting activity in lipopolysaccharide stimulated B cells, they were inactive. On the contrary, the same constructs were transcriptionally active in some but not all B cell lines. Thus, E‐boxes display functional heterogeneity as transcriptional activators depending on host cell.