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Autoreactive Antibodies in Thymus and Spleen of Neonatal and Young Adult BALB/c Mice: Influence of Prenatal Tolerization
Author(s) -
ACHTNICH M.,
ZÖLLER M.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb02489.x
Subject(s) - spleen , microbiology and biotechnology , balb/c , immunology , antibody , autoantibody , antigen , biology , immune system
The repertoire of autoantibody‐producing B cells was evaluated in a collection of spleen‐ and thymus‐derived hybridomas from 6‐ and 2S‐day‐old BALB/c mice, which were untreated or prenatally tolerized with trinitrobenzenesulphonic acid (TNBS). MoAb were tested for their reactivity with TNP‐BSA and the autoantigens thyroglaobulin (TG), myoglobin (MG), actin (AC), cytochrome C (CY), collagen (CO), transferrin (TF), single‐stranded DNA (dsDNA), double‐stranded DNA (dsDNA), and bromelain‐lreated mouse red blood cells (BrMRBC). More than 10% of spleen cell (SC)‐derived MoA b from 6‐and 28‐day‐old control mice did bind to AC, ssDNA, dsDNA, MY, and TG, the frequency of MoA b reacting with MY, TG, And BrMRBC increasing with age. Thymus cell (TC)‐derived hybridomas contained autoreactive clones Eoo. but only few of them produced multireactive MoAb. MoAb from prenatally TNBS‐treated mice were more frequently autoreactive than MoAb from control mice, especially if derived from TC hybridomas. The most remarkable difference in the reactivity pattern as compared with MoAb from untreated mice consisted of a significant increase in the frequency of TG‐, My‐, ssDNA‐ and above all of dsDNA‐reactive MoAb, all TC‐derived multireactive MoAb binding to dsDNA. The differences in autoreactivily between MoAb from prenatally untreated and TNBS‐treated mice as well as age‐ and organ‐related variations support the interpretation that part of the repertoire of naturally activated B cells is not random but is influenced by and responding to the available panel of self antigens.