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Characterization of Nickel‐Specific T Cell Clones
Author(s) -
SILVENNOINENKASSINEN S.,
POIKONEN K.,
IKÄHEIMO I.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01791.x
Subject(s) - cd8 , biology , t cell , monoclonal antibody , microbiology and biotechnology , receptor , flow cytometry , antibody , secretion , t lymphocyte , gene , immune system , immunology , genetics , biochemistry
Nickel is the major cause of metal‐induced allergic dermatitis. Twelve nickel‐specific T cell clones were used to investigate the cellular immune reactions occurring in nickel sensitivity. The selection between the alternative T cell receptors α and βγδ and two alternative Vβ genes (Vβ5 and Vβ8) were studied to see if nickel induces a selective pressure for clones bearing particular genes. Cell surface markers were studied by monoclonal antibodies and flow cytometry. Soluble mediators were measured by an ELISA method. The clones used T cell receptor αβ genes but did not use Vβ5 or Vβ8. They were T helper clones with a primed memory marker (CD3 + CD4 + CD8 − CD45RO + ) and carried HLA‐DR. None of the clones secreted IL‐1α, all of them secreted IL‐2 receptor. Four clones secreted IL‐lβ, six IL‐4 and seven IL‐6, the peaks in IL‐2Rand IL‐6 secretion preceding 1L‐4 secretion. The clones helped immunoglobulin synthesis. The clones from late effector phase of the nickel allergic reaction favours the use of T cell receptors αβ genes. Nickel‐specific clones were phenotypically indistinguishable but differed in soluble mediators produced.