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CD5 + B‐Lymphocytes are as Dependent on T‐Helper Cells and as Responsive to T‐Suppressor Cells in Pokeweed Mitogen‐Stimulated Activation as Conventional CD5 − B‐Lymphocytes
Author(s) -
JONES B. M.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01786.x
Subject(s) - cd5 , pokeweed mitogen , biology , microbiology and biotechnology , cd8 , b cell , antibody , immunology , antigen , in vitro , biochemistry , peripheral blood mononuclear cell
Pokeweed mitogen (PWM)‐stimulated cultures were established with varying numbers of CD4 + or CD8 + cells and CD5 + immunoglobulin‐secreting cells were subsequently identified using a combination of haemolytic plaque formation and rosetting wilh immunobeads coated with anti‐Leu‐1. Il was found that CD5 + and CD5 + B‐cells required similar numbers of CD4 + cells for half‐maximum plaque‐forming cell (PFC) induction and that 50% suppression of both CD5 + and CD5 − PFC occurred with similar numbers of CD8 + cells. Suppression of both CD5 + and CD5 − PFC could occur when T‐helper signals were supplied by a PWM‐stimulated culture supernatant, indicating that both subsets could be directly suppressed by CD8 + cells rather than by indirect suppression of T‐helper cells. It is concluded that CD5 + B‐cells are as responsive to T‐regulatory influences as conventional CD5 − B‐cells.