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Differential Effects of Interferon‐γ and Low Molecular Weight BCGF on Growth of Human B Lymphocytes; Interferon‐γ Prolongs the Increased c‐MYC mRNA Levels After Activation
Author(s) -
LØMO J.,
SMELAND E. B.,
STOKKE T.,
HOLTE H.,
FUNDERUD S.,
BLOMHOFF H. K.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01783.x
Subject(s) - transferrin receptor , messenger rna , cell cycle , interferon , biology , cell growth , receptor , cell , transferrin , endocrinology , microbiology and biotechnology , medicine , gene , immunology , biochemistry
We have characterized the growth‐stimulating effect of Interferon‐γ (TFN‐γ) on various parameters of B cell growth, and compared the effects with those of low molecular weight B cell growth factor (lmw BCGF). We have found that IFN‐γ did not affect early changes induced by anti‐μ, like initial calcium‐flux and rise in mRNA‐ and protein levels of the proto‐oncogene c‐myc measured at 3 h. On the other hand, IFN‐γ enhanced the effect of anti‐μ on parameters measured later in the GI phase of the cell cycle, such as expression of the transferrin receptor and general transcriptional activity, measured as an increase in 7‐amitioactinoiTiycin D binding. In particular, whereas the c‐myc levels in anti‐μ‐treated cells peaked al 3 h and then gradually declined. IFN‐γ together with anti‐μ maintained the c‐myc levels at 24 h at approximately the same levels as seen at 3 h. Overall, lmw BCGF had a more potent effect on the parameters affected by IFN‐γ, correlating with stronger enhancement of DNA synthesis. However, in contrast to IFN‐γ lmw BCGF did not affect anti‐μ‐induced c‐myc mRNA levels. Thus this study has revealed differences between two B cell growth factors in effects on B cell cycle parameters.