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A Monoclonal IgM Antibody to a Methylcholanthrene‐Induced Tumour
Author(s) -
GIGLIOTTI D.,
TENEBERG S.,
ANDFRSSON R.,
ÅNGSTRÖM J.,
KARLSSON K.A.,
WIGZELL H.,
HANSSON M.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01781.x
Subject(s) - methylcholanthrene , monoclonal antibody , antibody , antigen , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , carcinogen
A monoclonal IgM antibody, H17, has been obtained from rats immunized with mouse fibrosarcoma cells from an in vitro established methylcholanthrene(MCA)induced tumour. H17 shows specific and very selective binding to α‐N‐acetylgalactosamine (GalNAcα) when tested for reactivity to a panel of glycolipids. It cross‐reacts with GalNAcα on the Forssman antigen extracted from dog small intestine, but not from the human blood group A determinant, a finding not commonly observed among antibodies with this specificity. Despite its specificity. H17 does not react with TA3‐Ha, a mouse mammary adenocarcinoma, known to express the Tn antigen (GalNAcα‐O‐Ser/Thr). The uniqueness of HI7 probably relates to the fact that it has been generated against an MCA‐induced tumour rather than against the pure saecharide itself, Minimum energy conformation structures of different GalNAcα containing saecharide molecules were computer modelled lo allow a plausible interpretation of the accessible site of GalNAcα for successful interaction with the H17 para tope as compared lo other GalNAcα binding antibodies.