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Enhancement of IgE Synthesis in the Human Myeloma Cell Line U‐266 with an IgE Binding Factor from a Human T‐Cell line
Author(s) -
NILSSON G.,
JERNBERG H.,
HELLMAN L,
AHLSTEDT S.,
NILSSON K.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01596.x
Subject(s) - immunoglobulin e , cell culture , multiple myeloma , immunology , medicine , biology , antibody , genetics
An IgE‐binding factor(s) (IgE‐BF(s)) was partially purified from the supernatant of human HTLV‐II carrying T‐cell line MO, This IgE‐BF(s) was shown to increase the IgE synthesis in the human myeloma cell line U‐266, but did not affect its viability or growth. The of effect of the IgE‐BF(s) was dose‐dependent and selective for IgE protein synthesis as β 2 ‐microglobulin synthesis in the U‐266 and the immunoglobulin production in the U‐1958 IgG‐secreting human myeloma cell line were unaffected. The IgE‐BF(s) increased the production of the c heavy chain but not the X light chain production. The IgE‐BF(s) was distinct from IL‐1β. IL‐3, IL‐4, IL‐5, lL‐6, TNF‐α, IFN‐α ‐β, ‐γ, M‐CSF, and fragments of CD23.