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Stimulation of Peripheral Blood T Cells by an Activated T‐Cell Line: a Novel Human Autologous T‐T Lymphocyte Reaction
Author(s) -
DITZIANKADANOFF R.,
PARKS L.,
EVAVOLD B.,
QUINTANS J.,
SWARTZ T. J.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01595.x
Subject(s) - t cell , antigen presenting cell , t lymphocyte , antigen , immune system , biology , cytotoxic t cell , interleukin 21 , mixed lymphocyte reaction , natural killer t cell , microbiology and biotechnology , stimulation , secretion , immunology , in vitro , endocrinology , biochemistry
T lymphocyte interactions have generally been described between discrete functional subsets. In our investigation of murine T‐cell interactions we described a type of T‐T interaction termed the 'Syngeneic T‐T Lymphocyte Reaction’in which activated T‐cell clones stimulated the proliferation of resting T cells mainly through a mechanism involving cell to cell contact To investigate whether similar reactions occur in the human immune system we used the human autoreactive T‐cell line C.1 to stimulate peripheral T cells. Line C! cells, which are not transformed and do not secrete IL2. consistently caused proliferation of purified freshly isolated autologous peripheral human T cells as measured by a [ 3 H]‐thymidine incorporation assay. The proliferation was seen in both the CD4and CDS subsets and could be inhibited with anti‐DR and anti‐CD2 antibodies. The stimulation is not due to carryover of classical antigen‐presenting cells or to the C.1 line cells acting as antigen‐presenting cells. We propose that some activated T cells, probably by expression of a surface molecule, can stimulate resting T cells thereby allowing for antigen‐non‐specific augmentation of the immune response.