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Idiotypic Replica of an Anti‐Human Tumour‐Associated Antigen Monoclonal Antibody DNA Sequence Comparison between Ab1 and Ab3
Author(s) -
FLAMINI G.,
MARCHE P. N.,
CAZENAVE P. A.,
NATALI P. G.,
SICCARDI A. G.,
VIALE G.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01559.x
Subject(s) - epitope , biology , monoclonal antibody , microbiology and biotechnology , homology (biology) , glycoprotein , gene , glycoconjugate , antigen , glycolipid , antibody , genetics , biochemistry
2G‐3 is an anti‐anti‐idiotypic MoAb (Ab3) obtained upon immunization wiih a monoclonal Ab2 (A3B10), which behaves as the “internal image” of CaMBrl. a saccharidic epitope defined by MoAb MBrl (Abl), CaMBrI is expressed on glycoconjugates of the human mammary carcinoma cell line MCF‐7 und on normal and neoplastic mammary gland epithelial cells, Abl and Ab3. although exhibiting, in many respects, superimposable paratopic and idiotopic specificities, show a non‐identical fine immunoreactivity, since 2G‐3 has a preferential reactivity with the saccharidic epitope mounted on glycoproteins. while MBrl reacts with both glycoproteins and glycolipids, V‐region sequence analysis has shown that: (i) the VK genes employed belong to different families(VK 1 and VK 10); (ii) the J K2 segment is shared by the two L chains(thus a high degree of homology is observed between VK CDR3s): (iii) the VH genes employed derive from the same family VHIIBJ55K (but show CDR homology only in CDR2): (iv) different JM region genes are employed. These data together with the comparison of deduced secondary structure parameters give further evidence fur the possible production of similar combining sites using different VH and VL germ‐line genes.