Premium
Expression of the LFA‐1 β2 Integrin (CD11a/CD18) and ICAM‐1 (CD54) in Normal and Coeliac Small Bowel Mucosa
Author(s) -
SMART C. J.,
CALABRESE A.,
OAKES D. J.,
HOWDLE P. D,
TREJDOSIEWICZ L. K.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01550.x
Subject(s) - lamina propria , cd18 , coeliac disease , lymphocyte function associated antigen 1 , biology , integrin , cell adhesion molecule , cd11a , antigen , microbiology and biotechnology , cd8 , immunofluorescence , pathology , intercellular adhesion molecule 1 , immunology , epithelium , integrin alpha m , receptor , flow cytometry , medicine , antibody , biochemistry , disease , genetics
The leucocyte adhesion molecules (β2 integrins) comprise CD11 α‐chains and a common β‐chain (CD18), CD11a (leucocyte Junction‐associated antigen 1, LFA‐1) is expressed by most T cells, and is involved in antigen presentation by macrophages via its counter‐receptor, intercellular adhesion molecule (ICAM‐1, CD54), By criteria of double‐label immunofluorescence of cryostat tissue sections, virtually all lamina propria T cells of the normal small bowel were found to express LFA‐1 strongly. By contrast, only 30‐60% of intra‐epithelial lymphocytes (IEL) expressed detectable LFA‐1 most of which were LFA‐I weak and CD18 ‐ ICAM‐1 was expressed strongly only by vascular endothelium, in coeliac disease, there was a modest increase of diffuse ICAM‐1 expression in the lamina propria, mainly in the subepithelial zone, where ICAM‐1 + macrophages were occasionally seen. There was also a slight overall increase in CD11a expression by IEL, seen predominantly in surface epithelium and mainly by the CD4 + minority subset, but not by CD4 ‐ CD8 ‐ (TcR γδ + ) cells. These data suggest that the LFA‐1/ICAM‐1‐dependent antigen presentation pathway is of minor importance to IEL in the normal small bowel, and does not assume a major role in coeliac disease.