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The Role of CD45RA on Human B‐Cell Function: Anti‐CD45RA Antibody (Anti‐2H4) Inhibits the Activation of Resting B Cells and Antibody Production of Activated B Cells Independently in Humans
Author(s) -
MORIKAWA K.,
OSEKO F.,
MORIKAWA S.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01547.x
Subject(s) - microbiology and biotechnology , antibody , b cell , antigen , epitope , monoclonal antibody , naive b cell , chemistry , receptor , transferrin receptor , t cell , biology , immune system , antigen presenting cell , immunology , biochemistry
Anti‐CD45RA antibody defined by anti‐2H4 monoclonal antibody has been reported to split CD4 + T cells into two distinct subpopulations. CD45RA antigen is present on the surface of virtually more than 95% B lymphocytes in the purified tonsillar B‐cell preparations. We examined the role of CD45RA antigen on human B‐cell function using this antibody. The addition of anti‐2H4 to tonsillar B cells inhibited the proliferative response induced by Stapylocoocus aureus Cowan strain I(SAC) in a dose‐dependent manner, Kinetic analysis indicated that anti‐2H4 exerted its inhibitory effect when added within the first 24 h of culture initiation during a 72‐h culture period. Anti‐2H4 inhibited the transferrin receptor expression without interfering with the expression of the IL‐2 receptor on SAC‐stimulated B cells in a short‐term culture. Anti‐2H4 blocked the progress of SAC‐stimulated B cells from the G1 to S phase of the cell cycle. These events suggested that anti‐CD45RA MoAb inhibited the proliferative response by directly acting on B cells in the G1 phase. In addition anti‐CD45RA antibody also had a suppressive effect on early phase of B‐cell differentiation. This effect appeared to be independent of its suppressive effect on proliferation, because anti‐CD45RA did not inhibit the proliferative response of pre‐activated B‐cells with lymphokines. These studies suggested that the restricted epitope recognized by anti‐2H4 antibody may be directly involved in regulatory function on B cells.