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Antigen Processing and Presentation by Small and Large B Cells
Author(s) -
GONTIJO C. M.,
MÖLLER G.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01538.x
Subject(s) - antigen , b 1 cell , microbiology and biotechnology , spleen , b cell , naive b cell , biology , flow cytometry , antigen presentation , cd40 , antigen presenting cell , cell sorting , thymidine , ovalbumin , immunology , antibody , t cell , immune system , cytotoxic t cell , in vitro , biochemistry
We have investigated the ability of different cells from non‐immunized mice of the BALB/c strain to present antigen to two ovalbumin‐specific I‐A d ‐restricted T hybridomas. Lipopolysaccharide‐activated B‐cell blasts were found to be the most efficient antigen‐presenting cells. Purified small and dense splenic B cells also stimulated the hybridomas although not to the same extent as the activated blasts, but comparable to non‐fractionated spleen cells. Glutaraldehyde‐treated B cells failed to present antigen, whereas F(ab′)2 anti‐mouse IgM‐treated B cells exhibited markedly increased ability lo present antigen. Using (low cytometry, we further purified the resting B cells by sorting the small lymphocytes to ensure that the ability of these cells to activate the hybridomas was not due to contamination with large non‐resting B cells. The sorted small B cells retained the ability of antigen presentation. Their resting state was confirmed by the fact that they did not incorporate [ 3 H]‐thymidine as shown by autoradiographic analysis.