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Detection, Isolation and Functional Studies of CD25 + T Cells in Lymph Nodes Involved by B‐Cell Non‐Hodgkin's Lymphomas
Author(s) -
BONNEFOIX T.,
CLARET E.,
PICCINNI M.P.,
JACOB M.C.,
ZHENG X.,
SOTTO J.J.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01524.x
Subject(s) - lymph , isolation (microbiology) , b cell , lymphoma , biology , pathology , medicine , cancer research , immunology , antibody , bioinformatics
We searched for the presence of IL2 receptor (CD25) on T cells as an activation marker m lymph nudes involved by B‐cell non‐Hodgkin's lymphomas (B‐NHL). In 26 malignant lymph nodes studied, the number of CD25 + T cells among total T cells was usually low when assessed by immunofluorescence analysis (mean ± SD: 6.7%± 11.2%), but greatly increased when an immunomagnetic rosette method was used (mean±SD: 17.5% plusmn; 16.6%). In six cases. CD25‐/CD25/CD25 + cells were isolated by immunomagnetic separation, with a purity greater than 97%, for both populations. Expansion of CD25 ‐ /CD25 + T cells was obtained with IL2 and PHA, then conditioned media (CM) were prepared. No IL2 activity was found in CM from both CD25 ‐ /CD25 + T cells when tested on CTLL2 cells. BCGF and BCDF μ/γ activities were assayed on normal B cells stimulated with soluble or insolubilized anti‐μ antibodies (BCGF) or with Cowan I (BCDF). Results of production of all these activities were comparable for both populations, and thus do not favour the possibility that CD25 + T cells closely associated with malignant B‐NHL cells in lymph nodes may influence their proliferation (BCGF) or expression/secretion of heavy chain isotype (BCDF μ/γ).