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Different Activation States of Human Lymphocytes after Antibody‐Mediated Stimulation via CD3 and the α/β T‐Cell Receptor
Author(s) -
SCHLITT H. J.,
SCHWINZER R,
WONIGFIT K.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb03215.x
Subject(s) - stimulation , cd3 , antibody , receptor , t cell receptor , cytotoxic t cell , t cell , interleukin 2 , biology , peripheral blood mononuclear cell , microbiology and biotechnology , chemistry , cd8 , immunology , immune system , endocrinology , in vitro , biochemistry
BMA031 is an IgG2b antibody directed towards the human α/β T‐cell receptor that is able to induce proliferation of peripheral blood mononuclear cells independent of antibody cross‐linking. The proliferative response to BMA031 during the first 1 days of culture is usually of similar magnitude lo that induced by the IgG2a CD3 antibody OKT3 but decreases quickly afterwards. Stimulation by BMA031 induces no measureable IL‐2 release, very low expression of the IL‐2 receptor, and does not trigger cytotoxic effector function. However, cross‐linking of the antibody or addition of IL‐2 leads So enhanced and prolonged proliferation, strong IL‐2 receptor expression, and cytotoxic activity, features that are usually found after Stimulation by the IgG2a CD3 antibody OKT3 in soluble form. The stimulatory effect of BMA031 cannot be diminished by IL‐2 receptor blocking, whereas stimulation by OKT3 is strongly reduced Moreover, proliferation induced by BMA031 has lower sensitivity to inhibition by ciclosporin than OKT3. From these results two major conclusions can be drawn: (I) an IL‐2‐independent way of activation may be important for the short‐term proliferation of the T cells stimulated by BMA031 and (2) after stimulation by BMA031. cells reach a state of activation that is different from that induced by OK.T3. These differences arc most likely related to the different specificities of the antibodies, α/β TcR versus CD3, suggesting that different activation signals are triggered via CD3 and via the α/β TcR.