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Immunological Marker Analysis of Mitogen‐Induced Proliferating Lymphocytes Using Brd U Incorporation or Screening of Metaphases
Author(s) -
ADRIAANSEN H. J.,
OSMAN C.,
DONGEN J. J. M.,
BRFSSER J. H. F. M. WIJDENESDE,
TILBORG C. M. J. M. KAPPETIJNVAN,
HOOIJKAAS H.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb03211.x
Subject(s) - pokeweed mitogen , concanavalin a , peripheral blood mononuclear cell , cell growth , proliferation marker , biology , mitogen activated protein kinase , immunology , t cell , cd8 , microbiology and biotechnology , cell , splenocyte , immune system , signal transduction , biochemistry , in vitro
The proliferative effects of the mitogens phylohaemagglutinin (PHA). concanavalin A (Con A), pokeweed mitogen (PWM). and staphylococcal protein A (SpA) were investigated using two different methods which enable immunological marker analysis of proliferating cells: either surface marker labelling followed by Brd U incorporation or screening of metaphases after surface marker labelling. Therefore peripheral blood mononuclear cells from six healthy volunteers were stimulated with these four mitogens. Both PHA and Con A gave rise to more CD8 + than CD4 + proliferating cells. PHA, but not Con A, induced B‐cell proliferation as well. PWM mainly caused T‐cell proliferation. SpA also appeared to be a potent T‐cell mitogen in addition to its capacity to induce B‐cell proliferation. However, in contrast to the other mitogens SpA predominantly stimulated CD4 + cells.