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Inhibitory Effect of Mycoplasma‐Released Arginase
Author(s) -
CLAESSON M. H.,
TSCHERNING T.,
NISSEN M. H.,
LIND K.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb03204.x
Subject(s) - arginase , mycoplasma , arginine deiminase , antiserum , biology , microbiology and biotechnology , cell culture , arginine , fermentation , cell growth , cell , biochemistry , immunology , amino acid , antibody , genetics
Non‐fermenting mycoplasma species deplete culture media for arginine through arginase activity linked to their arginine deiminase pathway, resulting in proliferation arrest and cell death in mycoplasma‐contaminated cell cultures. The presence of only 2‐3 Mycoplasma (M.) arginini‐contaminated T cells in a one‐way allogeneic mixed‐lymphocyte culture (MLC) significantly inhibits development of cytotoxic T‐cell activity. Likewise, strong degrees of inhibition are observed after addition of nanogram doses of M. arginini extracts (MAE) to MLC or cell proliferation cultures. M. arginini‐induced cell inhibition can be reversed by addition of excess arginine to the culture medium. Antisera raised against non‐fermenting, but not against fermenting, mycoplasma species block the inhibitory effect of MAE. SDS‐PAGE separation of MAE disclosed a broad band at 60 kDa which contained arginase activity when assayed in MLC and cell proliferation culture. SDS PAGE followed by western blotting and reaction with antisera raised against non‐fermenting mycoplasma species demonstrated a band at 43 kDa common for these micro‐organisms.