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Gamma Interferon‐Induced Specific Binding of Tetanus Toxin on the GG2EE Macrophage Cell Line
Author(s) -
BLASI E.,
PITZURRA L.,
FUAD A. M. BURHAN,
MARCONI P.,
BISTONI F.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02922.x
Subject(s) - toxin , tetanus , clostridium tetani , macrophage , cell culture , immunofluorescence , interferon gamma , interferon , cell , biology , chemistry , microbiology and biotechnology , antibody , in vitro , immunology , biochemistry , vaccination , genetics
We have recently demonstrated that tetanus toxin (TT) selectively inhibited gamma interferon‐(IFN‐γ)‐induced, but not basal, lysozyme activity in the GG2EE macrophage cell line. By an indirect immunofluorescence technique, we show that tetanus toxin, as well as the BIIb tetanus toxin‐derived fragment, selectively binds to IFN‐γ‐treated cells but fails to bind to control cells. Moreover, BIIb fragment and TT share the same binding sites, as demonstrated by competition/ displacement experiments. These data indicate that IFN‐γ pretreatment results in the acquisition of tetanus toxin binding sites on GG2EE cells by a mechanism(s) yet to be identified.