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Phenotypical and Functional Differentiation of CD4 + CD45RA + Human T Cells Following Polyclonal Activation
Author(s) -
KRISTENSSON K.,
DOHLSTEN M.,
FISCHER H.,
ERICSSON P. O,
HEDLUND G.,
SJÖGREN H.O.,
CARLSSON R.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02917.x
Subject(s) - polyclonal antibodies , lymphokine , biology , microbiology and biotechnology , antigen , t cell , cellular differentiation , immunology , immune system , genetics , gene
Human CD4 + T cells differ in their expression of the leucocyte common antigen. Antibodies detecting certain forms (CD45RA and CD45RO) of this antigen have been used to identify and isolate Subpopulations of the CD4 + T cells, These isolated subsets have been shown to have different abilities concerning lymphokine production and provision of help to B cells for Ig production. When these T‐cell subsets were activated in vitro with polyclonal activators, the CD45RA + cells lost this market and gained the expression of CD45RO. This was true for all mitogens used in this report, i.e. accessory cell‐dependent stimulation with SFA and accessory cell‐independent activation with PMA or PHA. A correlation between proliferation and differentiation was observed, but this was probably not causative as stimulation with PMA in the absence of DNA synthesis resulted in the acquistion of CD45RO and loss of the CD45RA antigen. Moreover, cells proliferating vigorously for long periods of lime expressed both markers at significant levels, which suggests that proliferation did not automatically result in complete loss of the CD45RA marker. The phenotypical differentiation was associated with a functional differentiation which induced the stimulated cells’ability to act as helper cells For Ig production and to produce gamma interferon (IFN‐ γ ). The results obtained in this study support the contention that the CD45RO + cells are precursors of the CD45RO + cells and that die two subsets represent different maturational stages of the same lineage.