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Monocyte‐Mediated Suppression of IL‐2‐Induced NK‐Cell Activation
Author(s) -
HELLSTRAND K.,
HERMODSSON S.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02908.x
Subject(s) - percoll , serotonin , receptor , monocyte , biology , chemistry , endocrinology , microbiology and biotechnology , medicine , immunology , centrifugation , biochemistry
In the present study, the effects of serotonin on human natural killer (NK)‐cell responsiveness to interleukin 2 (IL‐2) was investigated. Concomitant treatment of human lymphocytes, enriched for NK effector cells by Percoll density‐gradient centrifugation, with serotonin and/or IL‐2 yielded a synergistic activation of NK‐cell cytotoxicity (NKCC) in the presence but not in the absence of monocytes. The monocyte‐dependent. regulatory effects of serotonin and/or IL‐2 were prosta‐glandin‐independent and could be reconstituted when monocytes, recovered by countercurrent centrifugal elutriation (CCE), were added to purified NK effector cells. The effects of serotonin on baseline and IL‐2‐activalcd NK cells were mimicked by the 5‐HT 1A receptor‐specific agonists 8‐OH‐DPAT and (+)‐ALK. Our data suggest that serotonin regulates NK‐cell responsiveness to IL‐2 via 5‐HT 1a receptors.