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Oestrogen Receptors in Macrophages
Author(s) -
GULSHAN S.,
McCRUDEN A. B.,
STIMSON W. H.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02820.x
Subject(s) - receptor , dissociation constant , dihydrotestosterone , chemistry , endocrinology , cell surface receptor , cell , cell culture , biology , medicine , microbiology and biotechnology , biochemistry , hormone , androgen , genetics
The presence of receptors for oestradiol‐17β and 5α‐dihydrotestosterone(5α‐DHT) in the human monocytic leukaemia cell line JIII and rat peritoneal macrophages was investigated using whole‐cell assays. For both cell types. high‐affinity binding species for oestrogen were detected, whereas no indication of specific binding was observed for 5α‐DHT. Analysis of the data according to Scatchard showed curved lines, indicating the presence of two different oestrogen‐binding species. The dissociation constant (K d ) values for the receptors of the rat peritoneal maorophages were calculated to be 1.4 × 10 ‐10 M and 3.3 × 10 ‐9 M, while for the JIII cells, the K d values were 8.7 × 10 ‐11 M and 2.5 × 10 ‐9 M. Sucrose‐gradient ultracentrifugation identified one oestrogenbinding species or 7.IS. The receptors had a relatively high affinity for diethylstilboestrol (DES) but did not bind to a monoelonal antibody specific for the classical oestrogen receptor, suggesting that oestrogen receptors in macrophages could be of a different type.