Premium
Immunological Typing of Acute Leukaemias by Rosetting with Immunomagnetic Beads: Comparison with Immunofluorescence Staining
Author(s) -
SKJØNSBERG C.,
BLOMHOFF H. KIIL,
GAUDERNACK G.,
FUNDERUD S.,
BEISKE K.,
SMELAND E. B.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02807.x
Subject(s) - antigen , immunofluorescence , monoclonal antibody , immunophenotyping , staining , bone marrow , peripheral blood mononuclear cell , haematopoiesis , antibody , biology , immunomagnetic separation , immunology , cd19 , microbiology and biotechnology , pathology , medicine , stem cell , in vitro , biochemistry , genetics
Immunological phenotyping of acute leukaemias is important for a more precise diagnosis with respect lo both cell lineage and maturation level. We have developed a rapid and reliable method for immunophenotyping, based on the use of magnetic monodisperse beads coated with monoclonal antibodies. After only a 10‐min incubation of immunomagnetic beads (1MB) with mononuclear cells isolated from bone marrow or peripheral blood, the percentage of rosetting cells can be counted in the microscope. A panel of 16 monoclonal antibodies against haematopoietic cell‐surface antigens was applied on 29 cases of acute myclogenic (AML) or lymphocytic (ALL) leukaemias, in order to compare immunological typing by immunomagnetic beads with immunofluorescence staining (IF). In all the cases tested, the two methods showed a virtually identical antigen distribution. The procedure described offers the advantages of being fast and simple to perform. Moreover, it has a high specificity and is easy to interpret in cases with low antigen expression.