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T‐Cell Recognition of HLA Class II Molecules Induced by Gamma‐Interferon on a Colonic Adenocarcinoma Cell Line (HT29)
Author(s) -
LUNDIN K. E. A.,
SOLLID L. M.,
BOSNES V.,
GAUDERNACK G.,
THORSBY E.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02794.x
Subject(s) - human leukocyte antigen , immunology , biology , t cell , interferon gamma , microbiology and biotechnology , cell culture , interferon , antigen , cancer research , cytokine , chemistry , immune system , genetics
HLA class II molecules may be induced on non‐lymphoid cells by gamma‐interferon (IFN‐ y ). We investigated if HLA class II molecules induced by IFN‐ y on the HT29 colonic carcinoma cell line are functional, i.e, if they may be recognized by allogeneic T cells. We found that IFN‐ y ‐treated HT29 (HT29 IFN ) cells could not induce primary proliferative responses of peripheral blood T lymphocytes, nor were they able to induce proliferation in T‐lymphocytc clones (TLC) specific for HLA class II molecules found on HT29 IFN . However, in the presence of exogenous interleukin 2 (lL‐2). 1 of 5 DQw8‐specific TLC proliferated when restimulated with HT29 IFN , and 3 of these 5 TLC could very effectively inhibit the growth of HT29 IFN , probably due to a cytoloxic effect. Both the proliferative response and the cytotoxicity were inhibited by anti‐DQ MoAb. We conclude that T cells may recognize HLA‐DQ molecules on non‐lymphoid cells, which may be of relevance for autoimmune diseases, graft‐versus‐host disease, and possibly for the recognition of malignant cells.