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The Vδ Gene Usage by Freshly Isolated T Lymphocytes from Synovial Fluids in Rheumatoid Synovitis: a Preliminary Report
Author(s) -
SIOUD M.,
KJELDSENKRAGH J.,
QUAYLE A.,
KALVENES C.,
WAALEN K.,
FØRRE Ø.,
NATVIG J. B.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02787.x
Subject(s) - synovitis , synovial fluid , rheumatoid arthritis , peripheral blood mononuclear cell , microbiology and biotechnology , synovial membrane , southern blot , dot blot , gene , polymerase chain reaction , in situ hybridization , immunology , northern blot , biology , medicine , pathology , messenger rna , genetics , alternative medicine , in vitro , osteoarthritis
Taking advantage ofthe polymerase chain reaction we have studied the usage of variable delta‐(Vδ)) region genes in freshly isolated synovial fluid T cells from patients with rheumatoid synovitis. Amplified mRNA from one patient with rheumatoid arthritis (RA) was cloned into an Smaleleaved pUC19 vector and colonies were screened with probes for three of the known human variable δ‐gene families (Vδ1. Vδ2. Vδ3). Of 10 clones, seven used Vδl. two Vδ2 and one Vδ3. This pattern of distribution is different from that of normal peripheral blood, where approximately 60% of Tγδ) cells are reported to use the Vδ2 gene. Furthermore, Northern blot hybridization analyses of mononuclear cells from two additional synovial fluids derived from another patient with RA and one with juvenile rheumatoid arthritis (JRA) also showed significant hybridization only with Vδ I. In summary, these preliminary results suggest a usage of Vδ gene families in Tγδ lymphocytes in synovial fluid of rheumatoid patients different to that found in normal peripheral blood.